Macro technique

This is an emerald-eyed tree frog from the Atlantic coast of Brazil. It posed very patiently for my group at the workshop. I used a Canon ring flash for the illumination because it simulates diffused light, my preferred type of lighting for wildlife photography and especially macro work. My settings were 1/200, f/32, and 800 ISO, and I used a 50mm macro lens with one extension tube. The two semicircles of light that emanate from the flash were reflected in the eyes of the frog, so I used the clone tool in Photoshop to eliminate the distracting reflections. I find when critically focusing on very small subjects, it's easiest to move back and forth with the camera in tiny increments, and the moment the subject is in focus I shoot. I actually turn the autofocus off.