Costa Rican glass frog

This is a spotted glass frog I photographed during one of my photo tours to Costa Rica.  I used a 100-400mm Canon zoom set to 312mm to make the background completely out of focus.  At the same time, I used a lens aperture of f/10 which gave me complete depth of field on the frog.  As usual, I prefer soft and diffused lighting for macro photography because too much contrast, with the characteristic black shadows, reduces detail.  I made the back of the camera — i.e. the plane of the digital sensor — parallel with the surface of the frog to help insure it was entirely sharp.  My other settings were 1/500 and 8000 ISO.  In post-processing, I used Topaz DeNoise AI to mitigate the noise.