I am conducting my semi-annual frog and reptile workshop this weekend. so I thought it would be appropriate to post one of my macro shots from a previous workshop. This is an Amazon milk frog, and it is one of the many species we always have at the workshop. The greater the magnification, the more depth of field is lost. My feeling about shooting small subjects is that the reason we do so is to show their fascinating detail, and therefore it's important to retain as much depth of field -- i.e. sharp focus -- as possible. That means that the smallest lens aperture is needed, and that in turn means you need enough light to make that happen. The last thing you want is for the shutter speed to be too slow because the pictures won't be sharp, and this is why the entire workshop is done with flash. A ring flash or a twin flash macro setup is ideal because the light envelopes the tiny subjects very similar to diffused light outdoors. I recommend to the workshop participants they use f/32 for all the shots.
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