I photographed this Madagascan moon moth with a flash for two reasons. First, this provided enough light so I could have complete depth of field at f/32. Second, I wanted a black background so the moth would really stand out against the strong contrast. The light fall-off, based on the inverse square law, made the detail behind the insect go dark. I made sure the back of the camera was as parallel as possible to insure everything was sharp from edge to edge because in macro photography, even with the smallest aperture, depth of field may not be as extensive as you want. I took this with my medium format film camera (the Mamiya RZ 67) before I went digital, and I used a 110mm normal lens, a Metz 60 CT1 flash, Fujichrome Velvia 50 transparency film, and a tripod.
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